Auramine O 🧪
Auramine O is a synthetic basic dye used mainly in biological staining and industrial coloring.
Key Points
Type: Basic (cationic) dye
Color: Bright yellow
Chemical nature: Diarylmethane dye
Solubility: Soluble in water and alcohol
Uses
🔬 Microbiology:
Used in fluorescent staining to detect acid-fast bacteria, especially Mycobacterium tuberculosis
Commonly used in Auramine–Rhodamine stain
🏭 Industry:
Dyeing of paper, textiles, leather
🔍 Laboratory diagnostics:
Preferred over Ziehl–Neelsen stain because it is more sensitive
Health & Safety
⚠️ Considered toxic
⚠️ Classified as a potential carcinogen
Proper protective measures are required during handling










